Utilizing Trichoderma Virens On Sago Hampas Bioconversion Used As Ruminant Feed

Abstract

Hampas sago bioconversion has the objective to transform the fibers into the crude protein through solid state fermentation with the aid of fungi. In this study, hampas sago has been fermented using Trichoderma sp inoculum, and it was isolated from the rubber tree and identified base on the sequence of ribosomal DNA internal transcribed own internal space (rDNA-ITS) region, 99% homologous with T.virens. Furthermore, T. Virens have been cultured on Potato Dextrose Broth, which is enriched with mineral solution used in solid fermentation inoculum static. Hampas sago is obtained from the refinery in Bogor and Meranti, each treatment 200 g hampas sago added with a solution of mineral 3% (v/w) and inoculated with 2% (v/w) T.virens culture. The substrate used was a combination of hampas sago (S) and bagasse (B) with the experimental design as follows: 100% S (A), 90% S and 10% B (B), and 80% S and 20% B (C). The best fiber degradation products is a combination of fiber-C (73.6%) are relatively better against A (68.2%) and B (68.2%). Meanwhile, crude protein formed respectively is B (39.75% DM), A (29.99% DM), and C (11.96% DM). The results of this study can be concluded that the combination of the substrate between hampas sago with baggase better than the solid fermentation substrate that contains only hampas sago.